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Around 95% of the body's serotonin is produced in the gut, and serotonin is a precursor to melatonin

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UPS-mediated degradation involves a cascade of E1 (activating), E2 (conjugating), and E3 (ligating) enzymes that attach ubiquitin chains-typically linked via lysine-48-to target proteins for recognition by the 26S proteasome

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